Nuclei Isolation Kit: Nuclei PURE Prep from MilliporeSigma

Product Specs
ItemNuclei Isolation Kit: Nuclei PURE Prep
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNUC201
Applicationscell analysis
Quantity1KT

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) A Global Transcriptional Atlas of the Effect of Sleep Deprivation in the Mouse Frontal CortexbioRxivNovember 30, 2023Kaitlyn Ford, Elena Zuin, Dario Righelli, Elizabeth Medina, Hannah Schoch, Kristan Singletary, Christine Muheim, Marcos G. Frank, Stephanie C. Hicks, Davide Risso, et al.sequencing Nuclei were extracted from mouse frontal cortical tissue using the Nuclei PURE Prep kit (NUC201–1KT, Millipore Sigma, Burlington MA, USA) with volumes reduced to a quarter of the recommended amount.

(2) DAP12 deficiency alters microglia-oligodendrocyte communication and enhances resilience against tau toxicityResearch SquareOctober 27, 2023Wenjie Chen, Hao Chen, Li Fan, Man Ying Wong, Man Ying Wong, Fangmin Foxe, Winston Foxe, Winston Nessim, Gillian Carling, Gillian Carling, et al.or at 4 °C. In brief, mouse brain tissue was placed in 1,500 μl of nuclei PURE lysis buffer (Sigma, NUC201–1KT) and homogenized with a Dounce tissue grinder (Sigma, D8938–1SET) with 15 strokes with pestle A

(3) Cell type-specific gene expression dynamics during human brain maturationbioRxivOctober 6, 2023Christina Steyn, Ruvimbo Mishi, Stephanie Fillmore, Matthijs B. Verhoog, Jessica More, Ursula K. Rohlwink, Roger Melvill, James Butler, Johannes M. N. Enslin, Muazzam Jacobs, et al.solution (Nuclei EZ Lysis Buffer [Sigma-Aldrich, NUC101] or Nuclei PURE Lysis buffer [Sigma-Aldrich, NUC201] with 1 mM dithiothreitol [DTT, Promega, P1171, US] and 0.1% Triton X-100 [Sigma-Aldrich, NUC201–1KT,

(4) Light-inducible T cell engagers trigger, tune, and shape the activation of primary T cellsProceedings of the National Academy of SciencesSeptember 26, 2023Morgane Jaeger, Amandine Anastasio, Léa Chamy, Sophie Brustlein, Renaud Vincentelli, Fabien Durbesson, Julien Gigan, Morgane Thépaut, Rémy Char, Maud Boussand, et al.Inc., CA). For NFAT translocation analysis, T cell nuclei were purified with the Nuclei isolation Kit (NUC201 Sigma-Aldrich), fixed, and permeabilized with the fix/Perm reagents (from 00-5523-00 eBioscience) and

(5) Divergent patterns of healthy aging across human brain regions at single-cell resolution reveal links to neurodegenerative diseasebioRxivAugust 1, 2023Megan F. Duffy, Jinhui Ding, Rebekah G. Langston, Syed I. Shah, Mike A. Nalls, Sonja W. Scholz, D. Thad Whitaker, Pavan K. Auluck, Stefano Marenco, J. Raphael Gibbs, et al.nuclei from human brain Nuclei were isolated using the Nuclei PURE Prep Nuclei Isolation Kit (Sigma #NUC201) per the manufacturer’s instructions with slight modifications. To streamline workflow and decrease

(6) Nuclear Translocation of LDHA Promotes the Catabolism of BCAAs to Sustain GBM Cell Proliferation through the TxN Antioxidant PathwayInternational journal of molecular sciencesMay 27, 2023Li Z, Gu Z, Wang L, Guan Y, Lyu Y, Zhang J, Wang Y, Wang X, Xiong J, Liu Y.Nuclear Isolation Isolation of nuclei was performed using a commercially available nuclei isolation kit (NUC201, Sigma Aldrich St. Louis, MO, USA). Briefly, GBM cells in the lysis medium were carefully placed on

(7) Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionActa neuropathologica communicationsMay 22, 2023Seeker LA, Bestard-Cuche N, Jäkel S, Kazakou NL, Bøstrand SMK, Wagstaff LJ, Cholewa-Waclaw J, Kilpatrick AM, Van Bruggen D, Kabbe M, et al.(cerebellum sample) and therefore it was removed from the experiment. The nuclei PURE isolation kit (Sigma, NUC201-1KT) was used to isolate nuclei: Samples were lysed in 200 µl lysis buffer (PURE lysis buffer containing

(8) Tumor type and cell type-specific gene expression alterations in diverse pediatric central nervous system tumors identified using single nuclei RNA-seqResearch SquareFebruary 24, 2023Min Kyung Lee, Nasim Azizgolshani, Joshua A. Shapiro, Lananh N. Nguyen, Fred Kolling Kolling, George J. Zanazzi, Hildreth Robert Frost, Brock C. Christensenfrozen tissues were isolated following the Nuclei Pure Prep nuclei isolation kit (Sigma-Aldrich, Catalog ID: NUC201) with some modifications. To summarize, ~10mg of tissue were washed with PBS to remove extraneous OCT

(9) Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylationNature communicationsDecember 2, 2022Li W, Long Q, Wu H, Zhou Y, Duan L, Yuan H, Ding Y, Huang Y, Wu Y, Huang J, et al.5. Nuclei isolation Nuclei of cells were isolated using the Nuclei Pure Prep Nuclei Isolation kit (Sigma, NUC201) following the instruction of the manufacturer. The cytoplasmic or nuclei fractions could be separated

(10) Microglial NF-κB drives tau spreading and toxicity in a mouse model of tauopathyNature CommunicationsDecember 1, 2022Chao Wang et al.4 °C. In brief, mouse brain tissue was placed in 1500 µl of Sigma nuclei PURE lysis buffer (Sigma, NUC201-1KT) and homogenized with a Dounce tissue grinder (Sigma, D8938-1SET) with 15 strokes with pestle A

(11) Signatures of copy number alterations in human cancerNatureJune 30, 2022Christopher D. Steele et al.estimation Fresh frozen tumour tissue was thawed on ice, dissected and homogenized with 500 µl of lysis buffer (NUC201-1KT, Sigma). Following the release of single nuclei, samples were centrifuged, and the resulting precipitate

(12) Ferroptosis is essential for diabetic cardiomyopathy and is prevented by sulforaphane via AMPK/NRF2 pathwaysActa Pharmaceutica Sinica BFebruary 1, 2022Xiang Wang, Xinxin Chen, Wenqian Zhou, Hongbo Men, Terigen Bao, Yike Sun, Quanwei Wang, Yi Tan, Bradley B. Keller, Qian Tong, et al.performed as previously described30,31. Nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma–Aldrich, Huntington, MA, USA). Antibodies against ferritin heavy chain (1:1000), KEAP1 (1:1000),

(13) A robust method to isolate Drosophila fat body nuclei for transcriptomic analysisFly (Austin)January 1, 2022Vanika Gupta, Brian P. Lazzaroof WSB. We further removed cellular debris using sucrose gradient centrifugation. Using Sigma kit (NUC201-KT), we followed the protocol using instructions provided by 10X Chromium [12]. The protocol is as follows:

(14) Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling PathwayOxidative medicine and cellular longevityJanuary 1, 2022Gao Y, Hu M, Niu X, Li M, Xu L, Xiao Y, Zhang J, Wang H, Li L, Chu B, et al.collected as total proteins. Nuclear extracts were prepared using a nuclear isolation kit (cat. no. NUC201, Sigma-Aldrich, USA). Protein concentration was measured with the BCA Protein Assay Kit (Solarbio),

(15) Nuclei isolation from surgically resected human hippocampusSTAR ProtocolsDecember 17, 2021Fatma Ayhan, Connor Douglas, Bradley C. Lega, Genevieve Konopkaassays,Critical commercial assays,Critical commercial assays ,, Nuclei PURE Prep Isolation Kit,Sigma,Cat# NUC201-1KT RNA Screen Tape,Agilent Technologies,Cat# 5067-5576 Chromium Single Cell 3′ kit,10× Genomics,Cat#1000153 ,, Biological

(16) NogoA-expressing astrocytes limit peripheral macrophage infiltration after ischemic brain injury in primatesNature CommunicationsDecember 1, 2021Anthony G. Boghdadi et al.and tight pestles, 25 cycles each, in 2 mL ice-cold lysis buffer (Nuclei PURE Lysis buffer (Sigma #NUC201-1KT) + 0.1% NP40 (Invitrogen #FNN0021) + RNAse inhibitor (Invitrogen #10777-019). Following homogenization,

(17) Overexpressing low-density lipoprotein receptor reduces tau-associated neurodegeneration in relation to apoE-linked mechanismsNeuronAugust 4, 2021Shi Y, Andhey PS, Ising C, Wang K, Snipes LL, Boyer K, Lawson S, Yamada K, Qin W, Manis M, et al.130-107-677 RNeasy Mini Kit,QIAGEN,Cat# 74104 Nuclei Isolation Kit: Nuclei PURE Prep,Sigma-Aldrich,Cat# NUC201 RNA-to-cDNA kit,Applied Biosystems,Cat# 4387406 DC Protein Assay Kit I,BIO-RAD,Cat# 5000111 Deposited

(18) Single-nucleus chromatin accessibility and transcriptomic characterization of Alzheimer's diseaseNature geneticsAugust 1, 2021Morabito S, Miyoshi E, Michael N, Shahin S, Martini AC, Head E, Silva J, Leavy K, Perez-Rosendahl M, Swarup V.removed cellular debris by preparing a sucrose gradient (Nuclei PURE Prep Nuclei Isolation Kit, Cat#NUC201-1KT, Sigma). Nuclei were spun at 13,000 g for 45 minutes at 4°C and then washed once and filtered before

(19) Molecular characterization of projection neuron subtypes in the mouse olfactory bulbeLifeJuly 22, 2021Sara Zeppilli, Tobias Ackels, Robin Attey, Nell Klimpert, Kimberly D Ritola, Stefan Boeing, Anton Crombach, Andreas T Schaefer, Alexander Fleischmanndissociate single nuclei, Nuclei PURE Prep was used according to the manufacturer instructions (Sigma, cat# NUC201-1KT) with some modifications. The minced tissue was gently homogenized in 2.75 ml Nuclei PURE Lysis

(20) Resolving cellular and molecular diversity along the hippocampal anterior-to-posterior axis in humansNeuronJuly 1, 2021Fatma Ayhan, Ashwinikumar Kulkarni, Stefano Berto, Karthigayini Sivaprakasam, Connor Douglas, Bradley C. Lega, Genevieve KonopkaSucrose Cushion Solution and Nuclei PURE Sucrose Cushion Buffer from Nuclei PURE Prep Isolation Kit (#NUC201-1KT, Sigma Aldrich). Nuclei PURE 2M Sucrose Cushion Solution and Nuclei PURE Sucrose Cushion Buffer

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Nuclei Isolation Kit: Nuclei PURE Prep from MilliporeSigma

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Nuclei Isolation Kit: Nuclei PURE Prep from MilliporeSigma

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